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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP384144AUDYeast1mgQuote
EP384144AUDE. coli1mgQuote
BP384144AUDBaculovirus200ugQuote
MP384144AUDMammalian Cell200ugQuote

Protein Information

SpeciesAcinetobacter baumannii (strain ATCC 17978 / NCDC KC 755)
UniProt IDA3M1S9
Gene NameleuD; Locus:A1S_0418
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-215
Expression Tag6xHis
Purity>90%
AA SequenceMKAYTVEQGIVAPLDRANVDTDLIIPKQFLKSIKRTGFGDNLFDELRYLDEGYPGQDNSV RPKNPDFVLNQPRYQGATVLIARTNFGCGSSREHAPWALNEYGFRTVIAPSFADIFFNNC FKNGMLPVILPEDIVDQLFKECAAQEGYQLTIDLAAQEVRTPTGEAFKFEVDPFRKHCLL NGLDDIGLTLQNADAIRAYEEKTKQVRPWVFQEIN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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