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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386523AUDYeast1mgQuote
EP386523AUDE. coli1mgQuote
BP386523AUDBaculovirus200ugQuote
MP386523AUDMammalian Cell200ugQuote

Protein Information

SpeciesAcinetobacter baumannii (strain ATCC 17978 / NCDC KC 755)
UniProt IDA3M1S8
Gene NameleuC; Locus:A1S_0417
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-472
Expression Tag6xHis
Purity>90%
AA SequenceMAGKTLYDKLWDDHVVTQRDDGSCLLYIDRHLLHEVTSPQAFEGLQLAGRQPWRLSANVA TPDHNVPTSKKERDQGIAGIEDDTSRIQVQTLDDNCKAFNIVEFGINDIRQGIVHVVGPE QGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLVQKKSKNMLVRVDGVL GKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGARVGMVA VDEKTIEYVKGRSYAPKGEQWDQAVAYWNTLHSDDDAVFDAVVELNGTEIEPQVSWGTSP EMVIPVSKAVPTLEQAKDDVQRNDWTRAYQYMGLNAGQALADIQLDRVFIGSCTNSRIED IRAAAEVVKGRKVAPSIKQAMIVPGSGLVKQQAEKEGLDKIFLEAGFEWREPGCSMCLAM NADKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAAIAGHFVDVRSF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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