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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386872BPUYeast1mgQuote
EP386872BPUE. coli1mgQuote
BP386872BPUBaculovirus200ugQuote
MP386872BPUMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia pseudomallei (strain 668)
UniProt IDA3NM77
Gene NameleuC; Locus:BURPS668_A2454
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKLAQRPVWRISANLAV SDHNVPTTDRSHGIADPVSKLQVDTLDANCDAYGITQFKMNDVRQGIVHIIGPEQGATLP GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGQLPRGCTA KDIVLAIIGRIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTV EYLKGRPFVPTGAEWDQAVEYWKTFRSDEGAQFDRVVELDAAQIVPQVTWGTSPEMVTSI DGRVPDPEREKDPVKRDAMERALAYMALAPNTPIEAIKVDKIFIGSCTNARIEDIRAAAY VVKKLNRRVAPNVRLAMVVPGSGLVKAQAEREGLDKVFTEAGFEWREPGCSMCLAMNADR LEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAIEGHFVDIRRLG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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