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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388814STOYeast1mgQuote
EP388814STOE. coli1mgQuote
BP388814STOBaculovirus200ugQuote
MP388814STOMammalian Cell200ugQuote

Protein Information

SpeciesShewanella loihica (strain ATCC BAA-1088 / PV-4)
UniProt IDA3QIN8
Gene NameleuC; Locus:Shew_3470
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMAKTLYEKVWDSHIVAAPEGEAPLIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFAT MDHNTSTKSASLDALSPMARTQVETLAQNCKEFGVRLYDIHHKNQGIVHVMGPELGITLP GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQLKAKTMKIEVRGLVADGITA KDIVLAIIGKIGMDGGTGYVVEFCGEAIAALSMEGRMTVCNMAIEMGAKAGMIAPDQTTI DYLEGREFAPKGEAWQQAVAAWKALKSDEDAMFDAHVVLEASDIAPQLTWGTNPGQVVAI DQCVPNPEDETNPTVKASIEKALDYVALTPGTQMTDVSINKVFIGSCTNSRIEDLRSAAQ QAKGRKVAAGVTAIVVPGSGLVKEQAEAEGLDKIFIDAGFEWRLPGCSMCLAMNDDRLEA GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAIAGHFVDIRKPY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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