Request QuoteCatalog Number: xP383053PZGSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP383053PZGYeast1mgQuote
EP383053PZGE. coli1mgQuote
BP383053PZGBaculovirus200ugQuote
MP383053PZGMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9515)
UniProt IDA2BUN7
Gene NameleuC; Locus:P9515_02891
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMSQDTLFDKVWELHKVANLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPNRTVA TVDHIVPTDNQNRPFRDNLAEQMIDTLEKNCFEHKIKFFNIGSGKQGIVHVVAPELGLTQ PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLASQTIAMNKLKVRQIWCENKLSNGIY AKDLVLHIINHLGVKAGVGYAYEFAGPAISALSMEERMTICNMSIEGGARCGYINPDEKT FSYIKDKLCSPQNENWDKAIKWWKSLQSNENSIFDDVIKIDASRVEPTVTWGITPGQSIG ISQKIPSLGEIHPNDQFIAGEAYEYMGFKPGQPIKDTPIDVCFIGSCTNGRISDLRVAAR VLGNHKIAKNIKAFVVPGSERVAKEAKEEGLDKVFIDAGFQWREPGCSMCLAMNSDKLIG NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAIKGKVSDVRDFLNK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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