Request QuoteCatalog Number: xP382890LNDSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP382890LNDYeast1mgQuote
EP382890LNDE. coli1mgQuote
BP382890LNDBaculovirus200ugQuote
MP382890LNDMammalian Cell200ugQuote

Protein Information

SpeciesLactococcus lactis subsp. cremoris (strain MG1363)
UniProt IDA2RKR3
Gene NameleuC; Locus:llmg_1284
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-458
Expression Tag6xHis
Purity>90%
AA SequenceMSGQTIFDKLWNQHVIAGQEGEPQLLYIDLHVIHEVTSPQAFQGLRDAGRSVRRRDLTYG TLDHNVPTKDIFNIQDLISKKQIDTFTKNVKEFGIPAEAHGGKGQGIVHMVAPESGRTQP GKIIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGVYS KDFILALIAKYGVDAGVGYAVEYTGNAISDLSMEERMTICNMSIEFGAKMGLMNPDEKTY DYVKGREYAPKDFDEAVSKWEKLVSDSDAVYDKVLTLDVSQLKPMVTWGTNPGMGLEFGE EFPKINDDLNYERAYQYMDLKPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIKGNHIA DGLTGIVVPGSRPVKRAAEELGLDKIFKDAGFEWREPGCSACLGMNPDQIPAYVHCASTS NRNFEGRQGHNARTHLCSPAMAAAAAISGKFVDVREIV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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