Request QuoteCatalog Number: xP311384DPXSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP311384DPXYeast1mgQuote
EP311384DPXE. coli1mgQuote
BP311384DPXBaculovirus200ugQuote
MP311384DPXMammalian Cell200ugQuote

Protein Information

SpeciesAzotobacter vinelandii
UniProt IDP96197
Gene NameleuB
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-360
Expression Tag6xHis
Purity>90%
AA SequenceMSKQILVLPGDGIGPEIMAEAVKVLQLANEKYQLGFVLGYDELGGAAVDKYGVPLADETL ERARRRCHPARRRRRSEMGRHRPGHPPGARPAENPFATGPVRQPAPGVLYPQLAEASTLR PEVVAGLDILIVRELTGGIYFGAPRESRLLANGERMAYDTLPYSESEIRRIAKVGFDMAR VRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDVVLSHMYVDNAAMQLVRAPKQFDVIV TDNMFGDILSDEASMLTGSIGMLPSASLDADNKGMYEPCHGSRRPDIAGKGIANPLATIL SVSMMLRYSFGQVEAANAIEQAVSKVLDQGLRTGDIWSEGCRKVGTAEMGDAVVAALATL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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