Request QuoteCatalog Number: xP310977MVZSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP310977MVZYeast1mgQuote
EP310977MVZE. coli1mgQuote
BP310977MVZBaculovirus200ugQuote
MP310977MVZMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium tuberculosis
UniProt IDP95313
Gene NameleuB; Locus:Rv2995c, MT3073; ORFs:MTV012.09
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-336
Expression Tag6xHis
Purity>90%
AA SequenceMKLAIIAGDGIGPEVTAEAVKVLDAVVPGVQKTSYDLGARRFHATGEVLPDSVVAELRNH DAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGIDFVVV REGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRRRKHLTLVHKTN VLTFAGGLWLRTVDEVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGDIITDL AAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLSHLGE HDAAARVDRAVEAHLATRGSERLATSDVGERIAAAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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