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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP305005SSQYeast1mgQuote
EP305005SSQE. coli1mgQuote
BP305005SSQBaculovirus200ugQuote
MP305005SSQMammalian Cell200ugQuote

Protein Information

SpeciesSynechocystis sp. (strain PCC 6803 / Kazusa)
UniProt IDP73960
Gene NameleuB; Locus:slr1517
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-362
Expression Tag6xHis
Purity>90%
AA SequenceMSQTYNVTLLPGDGIGPEIMAVAVAVLGKVADQFGFAFNFQEALIGGAAIDATGQPLPEA TLAQAKDSDAVLLAAIGGYAWDNLPRSQRPETGLLAIREGLGLFANLRPATIFPQLIDAS SLKREVVEGVDIMVVRELTGGIYFGKPKGIFETETGEKRGVNTMAYTVGEIDRIAKVAFE TARKRRGQLCSVDKANVLDVSQLWRDRVMAIAVDYPDVELSHLYVDNAAMQLVRSPRQFD TIVTGNLFGDILSDIAAMLTGSIGMLPSASLGSDGPGLFEPVHGSAPDIAGQDKANPLAQ VLSAAMMLRYGLDQPQAADRLEDAVKKVLEQGYRTGDILSPGTQLVGCRQMGEQLLSILD EM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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