Request QuoteCatalog Number: xP530064FHXSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP530064FHXYeast1mgQuote
EP530064FHXE. coli1mgQuote
BP530064FHXBaculovirus200ugQuote
MP530064FHXMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
UniProt IDO59391
Gene NameleuC; Locus:PH1726
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-380
Expression Tag6xHis
Purity>90%
AA SequenceMTLVEEILDAKAGEVVIRNVDLVYAHDGTMPLIIEAFNKVFNSVKARAYVFFDHVYPAPT VKVANLQKEIREFARRHGIPVVEGKGISHQLVVEMGLAEKSRIVVGGDSHTPTLGALGVF AVGMGATDVAVVLGLGKTWLRVPESVAVIFEGKPSRTTMAADVMIRLITSLRNYDMNYKA LEFFNVPFTADERLTLTNFSVEANAKTALIGEEYDGRNYIKEISFELSSLGPMVAKPFSP ANGVEVEKVEGTKIDQVFIGSCTNGRFEHIKRAAEILKGEEVAVRTIIGPASVNVYKRMV REGIVDILLDAGATILPPGCGPCLGRHMGVLGDKEVVLSTTNRNFRGRMGSPTAEIYLAS PLTAAVSALYGEITSPGGEV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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