Request QuoteCatalog Number: xP521890MVNSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP521890MVNYeast1mgQuote
EP521890MVNE. coli1mgQuote
BP521890MVNBaculovirus200ugQuote
MP521890MVNMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium leprae
UniProt IDO33117
Gene NameleuB; Locus:ML1691; ORFs:MLCB637.26
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-336
Expression Tag6xHis
Purity>90%
AA SequenceMKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREH DAILLGAVGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVV REGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNN VLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGE DEPAARLDQAVASYLATRGNGRFSTGEVGERIAAAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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