Request QuoteCatalog Number: xP524581EUKSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (LEU2)

Recombinant 3-isopropylmalate dehydrogenase (LEU2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP524581EUKYeast1mgQuote
EP524581EUKE. coli1mgQuote
BP524581EUKBaculovirus200ugQuote
MP524581EUKMammalian Cell200ugQuote

Protein Information

SpeciesPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
UniProt IDO59930
Gene NameLEU2
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-380
Expression Tag6xHis
Purity>90%
AA SequenceMSKRTFKIVILPGDGIGPEVVAEATRVLEVVSASSSDVEIKLETHDFGGCSIDKHGEPLT AATLEACKLADAILLGAIGGPKWGVNSKVRPEQALLALRKALGLYANIRPANFASDSLLA YSPLKPSVARGVDIIVIRELIGGAYFGERKELGARAQEDAAWDTMIYSVPEVQRITRSRR QVASPDPPLPVHSIDKANVLASSRLWRKVATETIQNEFPQLKLDHHLVDSASMLIVANPK KLNGVILTENLFGDILSDESSVIPGSLGLLPSASLAARPLWPNTAHRRSSPRRVYEPIHG SAPDIAGQGIANPIGTILSAAMLCGTRSVWRSPAKAIEAAVEGARRQGDRREGLRTADLG GSSKTKDIGHKVVEILKATL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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