Request QuoteCatalog Number: xP529524BXBSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529524BXBYeast1mgQuote
EP529524BXBE. coli1mgQuote
BP529524BXBBaculovirus200ugQuote
MP529524BXBMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Diuraphis noxia
UniProt IDO85072
Gene NameleuC
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMNKTLYEKIYDSHVVHSEKNGLSILYVDLHLLHEVTSPQAFESLRIKDRTVRQPKKTFAT MDHNVSTESKDINASGSMAKIQMQQLIKNCKEFHISLYDLNHPNQGIVHVISPEQGMTLP GMVIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFKNMKIEVIGKIGNFITA KDVILYIIGKIGSSAGTGYIIEFCGNVIKKMSMEERMTVCNMAIELGAKSGLIAPDETTY LYLKNKTYSPHGRNWQKAIEYWKTLKTDHNAIFDKVFTIDISNILPQVTWGTNPDQVIGI NEKIPDFTSFQNIVKKDLAKSACKYMDLKPGTYLTDITIDKVFIGSCTNSRIEDLRSASK ILKHNKISKNVKAIVVPGSGLVKRQAESEGLDKIFIEAGFEWRLPGCSMCLGMNNDRLSE NERCASTSNRNFEGRQGRNGRTHLVSPIMAALAALYGKFSNPNKLN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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