Request QuoteCatalog Number: xP529523BXESize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529523BXEYeast1mgQuote
EP529523BXEE. coli1mgQuote
BP529523BXEBaculovirus200ugQuote
MP529523BXEMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
UniProt IDO85066
Gene NameleuD; Locus:BUsg_PL7
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-207
Expression Tag6xHis
Purity>90%
AA SequenceMFKFIEHTGVVVPLNISNIDTDAIIPKQFLKKVNKIGFGKYLFHDWRFLDKNQLKINPNF ILNKSTYKNASILLTRENFGCGSSREHAVWSLLDYGFKVIIASSFSDIFYSNSFNNKLLL IVLENEDIDYLFDLVNTKIGLSFNVSLIHKKITVDNLDIPFQIDDFQRLCLVNNWDNNDL TMKIDHKIKLYEKNIFSFLLKREKFTS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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