Request QuoteCatalog Number: xP528315FOBSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528315FOBYeast1mgQuote
EP528315FOBE. coli1mgQuote
BP528315FOBBaculovirus200ugQuote
MP528315FOBMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces coelicolor (strain ATCC BAA-471 / A3 (2) / M145)
UniProt IDO86535
Gene NameleuD; Locus:SCO5554; ORFs:SC1C2.35
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-197
Expression Tag6xHis
Purity>90%
AA SequenceMEAFTKHTGRAVPLRRSNVDTDQIIPAHWLKKVTRDGFEDGLFEAWRKDPEFVLNRPERE GATVLVAGPDFGTGSSREHAVWALQNYGFKTVVSSRFADIFRGNSLKNGLLTVVLDQKTV DALWELVERDPQAEITVDLEAREVRAEGVTASFELDENSRWRLLNGLDDISITLQNEADI AAYEAKRPSFKPQTVQV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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