Request QuoteCatalog Number: xP529550FOBSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529550FOBYeast1mgQuote
EP529550FOBE. coli1mgQuote
BP529550FOBBaculovirus200ugQuote
MP529550FOBMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces coelicolor (strain ATCC BAA-471 / A3 (2) / M145)
UniProt IDO86534
Gene NameleuC; Locus:SCO5553; ORFs:SC1C2.34
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-476
Expression Tag6xHis
Purity>90%
AA SequenceMGRTLAEKVWDDHVVRRAEGEPDLLFIDLHLLHEVTSPQAFDGLRKSGRPVRRLDLTIAT EDHNTPTLDIDKPIADPVSRAQLETLRKNCADFGVRLHPLGDVEQGVVHVVGPQLGLTQP GTTVVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPLARPKTMAITVDGELPEGVTA KDLILAIIARIGTGGGQGYILEYRGEAIEKLSMEARMTICNMSIEAGARAGMIAPDETTF AYLQGRPHAPEGADWDAAVEYWKTLRTDDDAEFDAEVVIEAAELAPFVTWGTNPGQGAPL SAAVPDPASYEDASERFAAEKALEYMGLEAGQPLRSIQVDTVFVGSCTNGRIEDLRAAAE IVRDRKVADGVRMLVVPGSARVGLQAVSEGLDVVFKEAGAEWRHAGCSMCLGMNPDQLAP GERSASTSNRNFEGRQGKGGRTHLVSPQVAAATAVLGHLASPADLSAADVPTPAGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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