Request QuoteCatalog Number: xP527083FOBSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP527083FOBYeast1mgQuote
EP527083FOBE. coli1mgQuote
BP527083FOBBaculovirus200ugQuote
MP527083FOBMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces coelicolor (strain ATCC BAA-471 / A3 (2) / M145)
UniProt IDO86504
Gene NameleuB; Locus:SCO5522; ORFs:SC1C2.03
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-347
Expression Tag6xHis
Purity>90%
AA SequenceMSRSLNLAVIPGDGIGQEVVAEGLKVLSAVLPQDVKLETKEFDFGARRYHATGETLTDAD LDALKAHDAILLGAIGDPSVPSGVLERGFLLKLRFAFDHHVNLRPSKLLPGVATPLAGQP EIDFVVVREGTEGPYTGNGGTIRKGTEHEVATEVSVNTAYGVERVVRDAFARAQARPRKK LTLVHKNNVLTFAGHLWTNIFNKVAAEYPEVTTDYLHVDAATIFLVTDPARFDVIVTDNL FGDIITDLAAAVSGGIGVAASGNINPSGDFPSMFEPVHGSAPDIAGQGKADPTATVLSVA LLLRHLGYEDEAARIEDAVSADLGERGDLPARSTSEIGDTLAARVAG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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