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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP530263DNVYeast1mgQuote
EP530263DNVE. coli1mgQuote
BP530263DNVBaculovirus200ugQuote
MP530263DNVMammalian Cell200ugQuote

Protein Information

SpeciesAquifex aeolicus (strain VF5)
UniProt IDO67399
Gene NameleuD; Locus:aq_1398
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-168
Expression Tag6xHis
Purity>90%
AA SequenceMQMKFRGRVWKFGDNVDTDQIIPARYLNTSDPYELAKHVMEDSEHPEFAKEHKEGDIIVA GKNFGSGSSREHAPIAIKYSGVPVVIAKSFARIFFRNAINIGLPIVEAPEAVDEIEHGDE IEVDLEKGVIKNLRTGKEYQATKFPKELQDILKAGGLMAYAKEKLASK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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