Request QuoteCatalog Number: xP528398SXMSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (LEU2)

Recombinant 3-isopropylmalate dehydrogenase (LEU2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528398SXMYeast1mgQuote
EP528398SXME. coli1mgQuote
BP528398SXMBaculovirus200ugQuote
MP528398SXMMammalian Cell200ugQuote

Protein Information

SpeciesScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
UniProt IDO94114
Gene NameLEU2; ORFs:PICST_68561
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-373
Expression Tag6xHis
Purity>90%
AA SequenceMSTVTKTITVLPGDHVGTEICNEAIKVLEAIEQATPYQKIHFEFKHHLIGGAAIDSTGVP LPDDSLAAAKSSDAVLLGAVGGPKWGTGAVRPEQGLLKIRKELNLYANLRPCNFASDALL ELSPLKSEIVKGTNFTVVRELVGGIYFGERQEQEESADGESAWDTEKYSVAEVTRITRMA AFMALQHNPPLPIWSLDKANVLASSRLWRKTVDKVMKEEFPQLTIQHQLIDSAAMILVQS PTKLNGIVITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNSAFGLYEPCHGSAPDL PENKVNPIATILSVAMMLRLSLDSLKEAEALEEAVRQVLDSGVRTADLRGTNSTKEVGEA VVAAVTKILKDAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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