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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510408ESQYeast1mgQuote
EP510408ESQE. coli1mgQuote
BP510408ESQBaculovirus200ugQuote
MP510408ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DEW1
Gene NameleuD; Locus:PC1_3610
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-200
Expression Tag6xHis
Purity>90%
AA SequenceMAKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFHDWRFLDDAGQQPNPEF VLNKPHYKGASILLARENFGCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLLP VKLSDEEVDELFKLVDGQEGINFIVDLENQVVQAGSKSYPFEIDSFRRHCMINGLDSIGL TLQHEASITEYEKNQPAFLN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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