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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514797ESQYeast1mgQuote
EP514797ESQE. coli1mgQuote
BP514797ESQBaculovirus200ugQuote
MP514797ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DEW0
Gene NameleuC; Locus:PC1_3609
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMGKTLYQKLFEAHVVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRKVRQPGKTFAT MDHNVSTQTKDINASGEMARIQMQELIKNCAEFGVQLYDLNHPYQGIVHVIGPEQGMTLP GMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGDAPHGITA KDIVLAIIGKTGSAGGTGHVVEFCGKAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF NYLKGRQFAPKDANWDAAVAYWNTLKSDDDAQFDTVVTLDAAQIAPQVTWGTNPGQVIAV NQEIPNPDSFSDPVERASAAKALAYMDLQPGIKLTDVKIDKVFIGSCTNSRIEDLRAAAE IAKGRKVAAGVQAIVVPGSGPVKTMAELEGLDKVFIEAGFEWRLPGCSMCLAMNNDRLNP GERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAVTGRFADVRELN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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