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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505645EOWYeast1mgQuote
EP505645EOWE. coli1mgQuote
BP505645EOWBaculovirus200ugQuote
MP505645EOWMammalian Cell200ugQuote

Protein Information

SpeciesEubacterium eligens (strain ATCC 27750 / VPI C15-48)
UniProt IDC4Z1K1
Gene NameleuD; Locus:EUBELI_01367
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-164
Expression Tag6xHis
Purity>90%
AA SequenceMNEKFSGKVWVLGDDIDTDIIIPTEYLALKTVEDMKPYAFSPLRPELAGQIKPGDIIVAG KNFGCGSSREQAPEVIKALGIKCVIAKSYARIFFRNSINNGLLLIENADLHDEVTEGDTI DVEVNAKITHNGKAYPIASLPDNLVDILNAGGLVKAMRKLNGLD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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