Request QuoteCatalog Number: xP505158DWZSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505158DWZYeast1mgQuote
EP505158DWZE. coli1mgQuote
BP505158DWZBaculovirus200ugQuote
MP505158DWZMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
UniProt IDC4LJH3
Gene NameleuB; Locus:ckrop_1234
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-342
Expression Tag6xHis
Purity>90%
AA SequenceMKLAVIPGDGIGTEVTAEALKVMRAALAGSGESIETTDYDLGARRYLRNGELLTDDDLAS LREHDAILLGAVGDPRTVPSGVLERGLLLKMRFVLDHHVNLRPSVLYPGVTSPLSDPGKV DFIVVREGTEGLYAGNGGSIRVDTPHEVANETSVNTRYGVERVVRYAFDRAQERRKKLTL VHKTNVLVYAGGLWQRTVDQVAQEYLDVTVDYCHIDAATIYMVTDPSRFDVIVTDNLFGD IITDLAGAVTGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGKGIADPTAAILSGALLF RHLGKTDEADAIEAAVRNDVASRDGSIRTEEVGTRIANSLDS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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