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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505157DWZYeast1mgQuote
EP505157DWZE. coli1mgQuote
BP505157DWZBaculovirus200ugQuote
MP505157DWZMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
UniProt IDC4LJF0
Gene NameleuD; Locus:ckrop_1210
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-195
Expression Tag6xHis
Purity>90%
AA SequenceMEQFISHTGVGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFARWREDPDFVLNQDTYK NGSILVAGPDFGTGSSREHAAWALMDYGFRVVISARFADIFRGNAGKNGLVAAQMTEEDI ELIWKLLDEQPGRTMTVDLEERTVTVGDAVFGFDIDDHTRWRLMEGLDDIALTLRNEDAI TQYEKSRSPFMPRTI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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