Request QuoteCatalog Number: xP504938TOQSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504938TOQYeast1mgQuote
EP504938TOQE. coli1mgQuote
BP504938TOQBaculovirus200ugQuote
MP504938TOQMammalian Cell200ugQuote

Protein Information

SpeciesTolumonas auensis (strain DSM 9187 / TA4)
UniProt IDC4LAV5
Gene NameleuC; Locus:Tola_0680
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-465
Expression Tag6xHis
Purity>90%
AA SequenceMAKTLYQKVFEAHVVYEAAGETPIIYIDRHLVHEVTSPQAFDGLREKGRKVRRTDRTWAT MDHNVSTTSNDINASGEMARVQMQTLMKNTDDFGVPLYGLNHKWQGIVHVMGPEIGLTLP GTTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQSRAKTMKIEVTGKVGPGVTA KDIVLAIIGKTGTAGGTGYVVEFCGTAIQALTMEERMTVCNMAIELGAKAGMVAADQTTF DYLKGRPFAPKGADWDAAVEYWSTLKTDAGAKFDAELVLDGAAIEPQVTWGTNPGQVISI NTPIPAPEDFADPVARSSAQKALEYMALTAGQKLSDVKIDKAFIGSCTNGRIEDIRAAAA VAKGRKVAPGVQALVVPGSQQVKAQAEAEGLDKILTEAGFEWRLPGCSMCLAMNNDRLEP GERCAATSNRNFEGRQGRGGRTHLVSPAMAAAAAITGHFVDVREL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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