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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP498203BYPYeast1mgQuote
EP498203BYPE. coli1mgQuote
BP498203BYPBaculovirus200ugQuote
MP498203BYPMammalian Cell200ugQuote

Protein Information

SpeciesAnaerocellum thermophilum (strain DSM 6725 / Z-1320)
UniProt IDB9MLV3
Gene NameleuD; Locus:Athe_2100
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-166
Expression Tag6xHis
Purity>90%
AA SequenceMIFKGKAHKYYDNIDTDVIIPARYLNTSDPNELAKHCLEDLDKEFVNKVQKGDILVAGKN FGCGSSREHAPIAIKACGVSCVIAKSFARIFYRNAINIGLPIVECEEAVDGIEAGDEVEV DLVNGIIKNLTKGKEFKAKPFPEFMQNIMKAGGLIEFVKGELKKDA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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