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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP497456AWKYeast1mgQuote
EP497456AWKE. coli1mgQuote
BP497456AWKBaculovirus200ugQuote
MP497456AWKMammalian Cell200ugQuote

Protein Information

SpeciesAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY) )
UniProt IDB9MDK8
Gene NameleuC; Locus:Dtpsy_2582
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMGRTLYDKIFDEHVVHTEEDGTAVLYIDRHLVHEVTSPQAFEGLREAGRKVWRVSSIVAT ADHNTPTTGWERGYDGIADPISKEQIVTLDKNIQESGAAAFFPFLSKRQGIVHVIGPENG ATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNMLVQVNGKVAP GITAKDIVLAIIGKIGTAGGTGYTIEFAGEAIRDLSMEGRMTVCNMAIEAGARAGLVAVD DKTINYVKGRPLAPTGVEWDQAVAYWKTLHSDADAKFDAVVELNAAEIVPQVTWGTSPEM VLGIDAVVPDPDKEKDPSKRGAIERALTYMGLQPGKPMDDIFVDKVFIGSCTNSRIEDMR EAAAVVKKLGQKVAKNIKLAMVVPGSGLVKEQAEREGLDAIFKAAGFEWREPGCSMCLAM NADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIHGHFVDIRQFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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