Request QuoteCatalog Number: xP521140CZISize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (LEU2)

Recombinant 3-isopropylmalate dehydrogenase (LEU2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP521140CZIYeast1mgQuote
EP521140CZIE. coli1mgQuote
BP521140CZIBaculovirus200ugQuote
MP521140CZIMammalian Cell200ugQuote

Protein Information

SpeciesCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
UniProt IDO14429
Gene NameLEU2; Locus:CAGL0H03795g
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMAVTKTIVVLPGDHVGQEITEEAIKVLNAIQECRPDKVNFKFDHHLIGGAAIDATGVPLP DEALEASKKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELQLYANLRPCNFASDSLLDL SPLKPEIARGTDFVVVRELVGGIYFGERKEDEGDGVAWDSEKYSVPEVQRITRMAAFMAL QHNPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPQLTVQHQLIDSAAMILVKNPTHLN GIIITNNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKGKV NPVATILSAAMMLKLSLDLFEEGEIIEQAVKKVLDSGIRTADLKGTNSTTEVGDAVAKAV RELLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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