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Recombinant 3-isopropylmalate dehydratase (leu2)

Recombinant 3-isopropylmalate dehydratase (leu2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP517652SXVYeast1mgQuote
EP517652SXVE. coli1mgQuote
BP517652SXVBaculovirus200ugQuote
MP517652SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO14289
Gene Nameleu2; ORFs:SPAC9E9.03
Protein Name3-isopropylmalate dehydratase
Region Expressed1-758
Expression Tag6xHis
Purity>90%
AA SequenceMSPSVASPKTLYDKVWDSHVVDLQEDGTCLLYIDRHLIHEVTSPQAFEGLRTAGRKVRHP ELALATVDHNIPTDPRKDMKDIASFIHQPDSRTQVLALENNIKEFGLTYYGMNDRRQGIV HVIGPEQGFTLPGTTLVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTILQRKSKNMRI RVNGKLPEGIASKDLILHIIGVIGTAGGTGSVIEFCGEAIEGLSMEARMSMCNMSIEAGA RAGMIAPDATTFEYVKNRPLAPKGDDWEQAVAYWKTLRSDENAKYDIEVEINAADVLPTV TWGTSPQDVIPINGNIPDPAHVKDNVRAASIQRSLEYMGLKPNTSIVSYPIDKVFIGSCT NSRIEDLRLAAAVVKGRKVAANVKDAMIVPGSGLVKKMAEAEGLDQIFIEAGFDWREAGC SMCLGMNPDQLKPYERCASTSNRNFEGRQGAKGRTHLVSPAMAAAAAIKGHLCNVREFFG DVSNGSPSIITNKNYDPSHDVEGDIGLSVDDATDAVTDADGIATNVAGSVSSGSAGIPKF TVVEGIAAPLPMANVDTDKIIPKQFLKTIKRTGLGQFAFYEIRYDADGKEIPDFVLNREP YRHATVLVAHDNFGCGSSREHAPWALNDFGIRVIIAPSFADIFFNNCFKNGMLPIPTPIE QVNDMMKAAENQVKFSVDLVNQTITYGDKQVKFDVEPFRKHCLVNGLDDIGLTLQKETMI DAFEAAREENFPWMNIKRSRARLSPVKSNKQSSSRNDW
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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