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Recombinant 3-isopropylmalate dehydratase large subunit 1 (leuC1)

Recombinant 3-isopropylmalate dehydratase large subunit 1 (leuC1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP519785DOCYeast1mgQuote
EP519785DOCE. coli1mgQuote
BP519785DOCBaculovirus200ugQuote
MP519785DOCMammalian Cell200ugQuote

Protein Information

SpeciesArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
UniProt IDO28316
Gene NameleuC1; Locus:AF_1963
Protein Name3-isopropylmalate dehydratase large subunit 1
Region Expressed1-418
Expression Tag6xHis
Purity>90%
AA SequenceMAQTLVEKIFSKASGKEVKAGEFVMANIDLAMIHDITAPLAIKAFREILGSDAKVWDKSK VIMAFDHQVPADSVHAAENHKMLRKFAEEQGILNYDVKGGIAHQIMVENHVEPGMLIVGA DSHTCMYGALGAFATGIGSTDMGFVLAMGKLWFKVPESIRFNVHGKLEKHVYGKDIVLKL IGMVGADGANYKACIYSGEVVEKLGMSDRLTMCNMAIEMGGKAGIVEPDKTTLEYLKAMG RPYEGELLKSDEDAEFQEVELDVTGMEPQVAAPHRVDNVVGISEVEGTRVDQVFIGSCTN GRYEDLKIAAEILKGEKVASNVRLIVIPASHREYRRALKEGLIEIFVDAGALVEAPCCGP CMGGSFGLIASGEVSVSTSNRNFIGRQGSPEGKIYLVNPAVAAATAIYGEITDPRKIK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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