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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514500TLRYeast1mgQuote
EP514500TLRE. coli1mgQuote
BP514500TLRBaculovirus200ugQuote
MP514500TLRMammalian Cell200ugQuote

Protein Information

SpeciesTeredinibacter turnerae (strain ATCC 39867 / T7901)
UniProt IDC5BL66
Gene NameleuC; Locus:TERTU_2502
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMAGRTLYDKLWDDHVVSQRDDGSSLIYIDRHIVHEVTSPQAFEGLRIAGRKPWRTDSVIA TPDHNVPTTFNERASGVDGIADPISKIQVKTLDDNCDELGIVEFKINDNRQGIVHVVGPE TGGCLPGMTIVCGDSHTSTNGALGALSFGIGTSEVEHVLATQCLVAKKMKNMLIKVDGQL GQGVTAKDVVLAIIAKIGTAGGTGFAIEFGGDVFRAMSMEGRLTVCNMAIEAGARAGMVA VDEITLDYVKGRAFAPKGDHWAMAEARWRELHSDADAVFDEVVELDGAQIKPQVSWGTSP EMVLPVDGVIPDPAQESDGVKAAGVARALEYMGLTPGQKITDIAVDRVFIGSCTNSRIED IRAAAKVVAGKTKAASVKEAIVVPGSGAVKAEAEAEGLDKIFTAANIEWREPGCSMCLAM NADRLGEGEHCASTSNRNFEGRQGYGGRTHLVSPAMAAAAAISGHFVDIRDWQS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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