Request QuoteCatalog Number: xP512315EJOSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP512315EJOYeast1mgQuote
EP512315EJOE. coli1mgQuote
BP512315EJOBaculovirus200ugQuote
MP512315EJOMammalian Cell200ugQuote

Protein Information

SpeciesEdwardsiella ictaluri (strain 93-146)
UniProt IDC5B7R2
Gene NameleuC; Locus:NT01EI_0718
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMGKTLYQKLYDAHIVHQTPGETPLLYIDRHLVHEVTSPQAFDGLRAQRRRVRRPEHTFAT MDHNVSTQSRDIHACGEMARVQMETLMANCREFGVQLYDLHHPHQGIVHVIGPEQGMTLP GSTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQDRARTMQVVIQGRCAEGISA KDIVLAVIGHLGHAGGTGYVVEFCGPAVEALSMEGRMTLCNMAIELGAKAGLIAPDETTF AYLQGRPFAPQGAQWQQAVDHWRTLRSDADARYDRTVTLQADDIAPQVTWGTNPGQVIAI DTPIPSPASLSDPIARASAHKALAYMDLQPGTRMSDVAIDRVFIGSCTNSRIEDLRAAAA VARGRRVAPGVQAMVVPGSGPVKAQAEAEGLDKIFTEAGFEWRLPGCSMCLAMNNDRLGS GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVCGHFADIRQFQEPAHD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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