Request QuoteCatalog Number: xP513477BSTSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513477BSTYeast1mgQuote
EP513477BSTE. coli1mgQuote
BP513477BSTBaculovirus200ugQuote
MP513477BSTMammalian Cell200ugQuote

Protein Information

SpeciesBeutenbergia cavernae (strain ATCC BAA-8 / DSM 12333 / NBRC 16432)
UniProt IDC5C3D1
Gene NameleuD; Locus:Bcav_1574
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-212
Expression Tag6xHis
Purity>90%
AA SequenceMEKFTTHTGVGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDALFAAWRGNETFVLNQPAYT HGSVLVAGPDFGTGSSREHAVWALKDYGFRVVIASRFADIFRGNSGKQGLLAAQVAQDDV ELIWKVLENSPGTEVTVDLAAKQVTVADIVAPFQIDDYTRWRLLEGLDDIGLTLQHADEI TAFEATREPWRPLTLPAKHLPAVPITAARPVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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