Request QuoteCatalog Number: xP511670BSTSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511670BSTYeast1mgQuote
EP511670BSTE. coli1mgQuote
BP511670BSTBaculovirus200ugQuote
MP511670BSTMammalian Cell200ugQuote

Protein Information

SpeciesBeutenbergia cavernae (strain ATCC BAA-8 / DSM 12333 / NBRC 16432)
UniProt IDC5C2I9
Gene NameleuB; Locus:Bcav_1417
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-347
Expression Tag6xHis
Purity>90%
AA SequenceMTRSIDLAVVAGDGIGPEVVAEGLKVLDAALAGTDVTVRTTAYDLGAARWHATGETLTDE DLAALAQHDAILLGAIGDPGVPSGVLERGLLLKLRFAFDHYVNLRPSRLYPGVATPLAKG GDVDFVVVREGTEGPYVGNGGAIRVGTPHEIANEVSVNTAFGVERVVRDAFARADARPRK KLTLVHKHNVLVHAGHLWRRTVEAVGAEFPDVAVDYLHVDAATIFLVTDPARFDVVVTDN LFGDILTDLAAAITGGIGLAASGNINPDRAFPSMFEPVHGSAPDIAGQGKADPTATVLSV ALLLEHLGLTEQAARITAAVESDIVERGSAVRSTAEVGDALAARVAG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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