Request QuoteCatalog Number: xP517977MSRSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP517977MSRYeast1mgQuote
EP517977MSRE. coli1mgQuote
BP517977MSRBaculovirus200ugQuote
MP517977MSRMammalian Cell200ugQuote

Protein Information

SpeciesMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
UniProt IDO27441
Gene NameleuB; Locus:MTH_1388
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-329
Expression Tag6xHis
Purity>90%
AA SequenceMKSMKIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAV GEARATLFGAAGESAADVIVRLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTEDL YVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQKVTAVHKANVLKKTDGIFRD EFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGLA PSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQKT LAEGLVTPDLGGKLGTMEMAAEIARHLED
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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