Request QuoteCatalog Number: xP512471GFJSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP512471GFJYeast1mgQuote
EP512471GFJE. coli1mgQuote
BP512471GFJBaculovirus200ugQuote
MP512471GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D5L8
Gene NameleuC; Locus:GWCH70_2591
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMKPKTIIEKIWENHVVYREDGKPDLLYIDLHLVHEVTSPQAFEGLRQKGRKVRRPDLTFA TMDHNVPTVNRLVIEDEVARNQIAALERNCREFSIPLADLRSEEQGIVHVIGPELGLTQP GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLWQHKPKTLQIRINGKLGEGVTA KDVILAIIGRYGVDVGTGYIIEFTGEVIRNMSMEERMTICNMSIEAGARAGLVSPDETTF AYLRGRKYAPKGEEFEKAVERWRALATDEGAEYDKTIEIDASTIAPMVTWGTNPSMSTSI EGTVPYPEDFSSETEQKAVRRALEYMGLEPGTPITEIPVQHVFIGSCTNSRISDLREAAK IVKGKKVAKGVRALVVPGSQQVKKQAEEEGLAQIFIDAGFEWRDSGCSACLGMNPDIIPE GEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIYGHFVDVRKLQKEPVH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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