Request QuoteCatalog Number: xP509482GFJSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509482GFJYeast1mgQuote
EP509482GFJE. coli1mgQuote
BP509482GFJBaculovirus200ugQuote
MP509482GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D5L7
Gene NameleuD; Locus:GWCH70_2590
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-197
Expression Tag6xHis
Purity>90%
AA SequenceMKPFTVHKGKVAGIDRANIDTDQIIPKQFLKRIERTGFGKFLFYDWRYIDGEKPNPDFEL NRPENEGATILVANENFGCGSSREHAPWALQDYGFQAIIAPSFADIFYNNCLKNGLLPIR LAKQDVEYLLRESTKADYELTISLEDQRVYDDSGFERTFDIDPYRKQLLLKGWDEIDLTF VYEPYIAEYEKKHCPMS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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