Request QuoteCatalog Number: xP513529VEMSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513529VEMYeast1mgQuote
EP513529VEME. coli1mgQuote
BP513529VEMBaculovirus200ugQuote
MP513529VEMMammalian Cell200ugQuote

Protein Information

SpeciesVariovorax paradoxus (strain S110)
UniProt IDC5CSH5
Gene NameleuC; Locus:Vapar_1422
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMARTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTSPQAFEGLREAGRKLWRISSVVAT ADHNTPTTGWERGYEGIADPTSKEQVTTLDKNIAEFGAAAFFPFLSKRQGIVHVIGPESG ATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLGKKAKNMLVKVEGKLPF GCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRDLSMEGRMTVCNMAIEAGARAGLVAVD EKTIGYIKGRPLAPTGVEWDQAVAYWRTLQSDPDAAFDAVVELDATQIQPQVTWGTSPEM VVDINGRVPDPDKEKDASKRSAIERALVYMGLEPNKAMNDIFIDKVFIGSCTNSRIEDMR EAAAVVKKLGQKVAKNVKLAMVVPGSGVVKEQAEREGLDVIFKAAGFEWREPGCSMCLAM NADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDVRTFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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