Request QuoteCatalog Number: xP499090RLKSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP499090RLKYeast1mgQuote
EP499090RLKE. coli1mgQuote
BP499090RLKBaculovirus200ugQuote
MP499090RLKMammalian Cell200ugQuote

Protein Information

SpeciesRhodococcus erythropolis (strain PR4 / NBRC 100887)
UniProt IDC0ZXM4
Gene NameleuB; Locus:RER_24010
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-337
Expression Tag6xHis
Purity>90%
AA SequenceMKLAVIPGDGIGVEVTAEALKVLGKLVPDLETTEYDLGARRYNATGELLPADELDQIRQH DAILLGAIGDPRIVAPGILERGLLLNMRFQLDHHVNLRPAQLYPGALSPLAAQPEIDFVV VREGTEGPYTGNGGAIRVGTDHEIATEVSINTWFGAERVVRYAFALAQTRSKHLTLIHKT NVLSNAGAIWTRAIETVGAEYPDVETAYCHIDAATIYMVTDPSRFDVIVTDNLFGDIITD LAGAVTGGIGLAASGNIDASGTNPSMFEPVHGSAPDIAGKGIADPTAAILSAALLLRHLG REDDAARVEAVVAADLATRGEGPISTTEIGDRITAAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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