Request QuoteCatalog Number: xP507977BWTSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP507977BWTYeast1mgQuote
EP507977BWTE. coli1mgQuote
BP507977BWTBaculovirus200ugQuote
MP507977BWTMammalian Cell200ugQuote

Protein Information

SpeciesBrucella melitensis biotype 2 (strain ATCC 23457)
UniProt IDC0RM30
Gene NameleuD; Locus:BMEA_B0862
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-201
Expression Tag6xHis
Purity>90%
AA SequenceMDKFTKLTGVAAPLPIVNIDTDMIIPKDYLKTIKRTGLGKGLFAEMRFNEDGSENPDFVL NKPGYRKAQILVAGDNFGCGSSREHAPWALLDYGIRCVISTSFADIFYNNCFKNGILPIK VAQEDLDKLMDDASRGANATLTIDLETKQIHGPDGGTISFDLDDFKRHCLLNGLDDIGLT MEKAKSIDTFEAKNAEERPWA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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