Request QuoteCatalog Number: xP501982VEZSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501982VEZYeast1mgQuote
EP501982VEZE. coli1mgQuote
BP501982VEZBaculovirus200ugQuote
MP501982VEZMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain M66-2)
UniProt IDC3LR34
Gene NameleuC; Locus:VCM66_2414
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMSKAKTLYEKIYDAHVVVAAPGETPILYIDRHLVHEVTSPQAFDGLREKGRPVRQVSKTF ATMDHNVSTTTKDINASGEMARIQMQTLSKNCEEFGVTLYDINHKYQGIVHVMGPELGIT LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVRGKVAPGI TAKDIVLAIIGKTTAAGGTGYVVEFCGEAIRDLSMEGRMTVCNMAIELGAKAGLIAPDAT TFNYIKGRKFAPQGSDWDAAVDYWQTLKTDEDAQFDAVVTLEASEIKPQVTWGTNPGQVI AVDEPIPSPSQFADPVERSSAEKALAYMGLEAGKMLSDYKVDKVFVGSCTNSRIEDMRAA AAVAKGKKVASHVQALIVPGSEQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRL GPGERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRQF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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