Request QuoteCatalog Number: xP519965BXHSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP519965BXHYeast1mgQuote
EP519965BXHE. coli1mgQuote
BP519965BXHBaculovirus200ugQuote
MP519965BXHMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Thelaxes suberi
UniProt IDO31293
Gene NameleuC
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMGQTLYEKLYNSHIIYEDKNTLPIIYIDLHLLHEVTSPQAFESLKNKKRIVHAPIKTFAT MDHNVSTKTNKISASGKAAQIQMQQLINNCKDFNIKLYDLNHINQGIVHVMGPEQGLTLP GMTIVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQTLKQARSKTMKIGINGILKPYITA KDVILYIIKKVGTAFGTGYVVEFSGETIENLTMEGRMTICNMVIEMGAKSGIIAPDATTY KYLKNLPYAPKKEKWKNALEYWKNLKTDNDAKFDKIVKFDVSQIEPQITWGTNPSQTINI TESNPDPKKINNIIDKQATEKALKYMNLKPNQKMINLVVDKVFIGSCTNSRIEDLRIASK IIKNKKVAKTTKAIVVPGSKLVKIQAEKEGLDKIFINAGFEWRLPGCSMCLAMNDDKLNR GERCASTSNRNFEDRQGRGGRTHLVSPITAAAAAIFGYFVDIKNIL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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