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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503657DWXYeast1mgQuote
EP503657DWXE. coli1mgQuote
BP503657DWXBaculovirus200ugQuote
MP503657DWXMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) (Corynebacterium nigricans)
UniProt IDC3PFX5
Gene NameleuB; Locus:cauri_1136
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-339
Expression Tag6xHis
Purity>90%
AA SequenceMKLAVIGGDGIGPEVTAEALKVLRAVRQDVEVTDYDLGARRYLRNGELLSDADFASLREH DAILLGAIGAPGEVPPGVLERGLLLKMRFALDHHVNLRPSKLYPTSTSPLANPGDIDFVV VREGTEGLYCGNGGTLREGTEHEVASEVSQNTRFGVERVVRDAFQRAMGRRKHVTLVHKT NVLVNAGGLWQRTVNEVAQEFPEVTVDYNHIDAATIYMVTDPARYDVIVTDNLFGDILTD LAGAVTGGIGLAASGNIDASGANPSMFEPVHGSAPDIAGKGIADPTAAILSAAMLLRHLG DEENAVRIEEAVAADVSARGDAQARTTEIGDRIAAALSA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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