Request QuoteCatalog Number: xP503352BQFSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503352BQFYeast1mgQuote
EP503352BQFE. coli1mgQuote
BP503352BQFBaculovirus200ugQuote
MP503352BQFMammalian Cell200ugQuote

Protein Information

SpeciesBacillus anthracis (strain A0248)
UniProt IDC3P4Z8
Gene NameleuC; Locus:BAA_1489
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK DIILHLLSKYGVAVGTGYVMEFYGETIGTMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK LPEKHDVNDERAFSYMGLIPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGRKVKEG VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR NFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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