Request QuoteCatalog Number: xP496689BQQSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP496689BQQYeast1mgQuote
EP496689BQQE. coli1mgQuote
BP496689BQQBaculovirus200ugQuote
MP496689BQQMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain Q1)
UniProt IDB9IUZ2
Gene NameleuC; Locus:BCQ_1475
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK DIILHLLSKYGVAVGTGYVMEFYGETIQAMEMEERMTLCNMAIEGGAKAGIIAPDEKTVA YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK LPEKHDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEG VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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