Request QuoteCatalog Number: xP493301EPVSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP493301EPVYeast1mgQuote
EP493301EPVE. coli1mgQuote
BP493301EPVBaculovirus200ugQuote
MP493301EPVMammalian Cell200ugQuote

Protein Information

SpeciesCyanothece sp. (strain PCC 7425 / ATCC 29141)
UniProt IDB8HLN1
Gene NameleuC; Locus:Cyan7425_1136
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-468
Expression Tag6xHis
Purity>90%
AA SequenceMSKGTLFDKVWDLHTVATLPSGQTQLFIGLHLIHEVTSPQAFAMLKERGLKVLFPERTIA TVDHIVPTDNQARPFADSLAEEMMQALETNCQEHGIRFYNIGSGSQGIVHVIAPELGLTQ PGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLSLSKLKVRKIEVNGSLQPGVY AKDVILHIIRTLGVKAGVGYAYEYAGTTFEQMSMEERMTVCNMAIEGGARCGYVNPDAVT FAYLKEREFAPQTDWDTAQQWWQSIASDPDAVYDDVVVFDAADIAPTVTWGITPGQGIAV DQPLPLPEQLPASEQAIAEEAYQYMDLIPGQKLLGTKIDVCFIGSCTNGRISDLRAAAEI AQGRRVVPGLKAFVVPGSERVKQQAEAEGLDQIFQAAGFEWREPGCSMCLAMNPDKLQGR QISASSSNRNFKGRQGSATGRTLLMSPAMVAAAAVTGEVTDVRELLGQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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