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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511142DJQYeast1mgQuote
EP511142DJQE. coli1mgQuote
BP511142DJQBaculovirus200ugQuote
MP511142DJQMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
UniProt IDC6BSH0
Gene NameleuD; Locus:Desal_1584
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-164
Expression Tag6xHis
Purity>90%
AA SequenceMSIKGTAYRVGAHIDTDAIIPARFLVTTDPDELGANCMEGLEAGWIKRVKKNDIMVADEN FGCGSSREHAPISILGAGIPVVVAKSFARIFYRNGFNMGLILLEVGDDFEKLGDGDQLEV DAEKGEIKNVTTGETITCAPVPPFMKGILDCGGLVEYVKDRLSK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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