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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP494243FLKYeast1mgQuote
EP494243FLKE. coli1mgQuote
BP494243FLKBaculovirus200ugQuote
MP494243FLKMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus carnosus (strain TM300)
UniProt IDB9DMJ8
Gene NameleuC; Locus:Sca_1565
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-460
Expression Tag6xHis
Purity>90%
AA SequenceMGQTLFDKIWNRHVLTGKEGEPQLLYIDLHLIHEVTSPQAFEGLRIQKRPLRRPDLTFAT IDHNVPTEDIFNIRDEIAATQIKTLQNNCAEFDVKLFDNGTDQQGIVHMVGPEMGLTQPG KTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQSLWQTKPKNLLIDINGKLPQGVYAK DIILHLINQYGVDFGTGYALEFGGSTMRNLSMDERMTICNMAIEAGAKYGLVQPDETTLE YVKDRPYADLTPEKLEYWKTLYSDEDAQFDCVIELDVTQLEPQVTWGTSPEMGVSFSTPF PAIENINDERAYQYMGLKPGEKAEDIELGYVFLGSCTNARLSDLVEASKFVKGHQVHPNI TAIVVPGSRSVKHQAEALGIDKIFKDAGFQWREPGCSMCLGMNPDQVPAGVHCASTSNRN FEGRQGKGARTHLVSPAMAAAAAIEGRFIDVRNWEAVINA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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