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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP498076NFRYeast1mgQuote
EP498076NFRE. coli1mgQuote
BP498076NFRBaculovirus200ugQuote
MP498076NFRMammalian Cell200ugQuote

Protein Information

SpeciesNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
UniProt IDB9L6Y8
Gene NameleuC; Locus:NAMH_1751
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-425
Expression Tag6xHis
Purity>90%
AA SequenceMPMTITEKIFAEHIGREVKPGEIIMCDVDMTIGNDITTPISIRAFNESGAEKLAKPDNFA IVLDHFIPPKDIASANQAKINRDFAYKHDLKNFFDEKDMGIEHRLLPEKGLVIPGDVIIG ADSHTCTHGALGAFSTGMGSTDIAFCMITGKSWFKIPESIKVVFKGERGEHVYGKDLILE LIRRIGVDGALYKALEFTGEVIENLPMDDRMSLCNMAIEAGAKNGIVAYDKITEAFLEEV KKYNPLRSEPKIHYSDPDANYSQVIEIDVNKLEPLVAYPFLPSNGKPISQAVKDDIEIDQ VYIGSCTNGTLSDLRIAAEILKGKRVHRRVRLIVTPATQRIYKQAEHEGILDILIDAGAV VANPTCGACLGGYMGILGDGERAVATTNRNFRGRMGSRSSEVYLSNSAVAAASAIAGKIA DPRDL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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