Request QuoteCatalog Number: xP475973OEDSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP475973OEDYeast1mgQuote
EP475973OEDE. coli1mgQuote
BP475973OEDBaculovirus200ugQuote
MP475973OEDMammalian Cell200ugQuote

Protein Information

SpeciesOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
UniProt IDB6JAQ9
Gene NameleuC; Locus:OCAR_4273, OCA5_c02460
Protein Name
Region Expressed1-470
Expression Tag6xHis
Purity>90%
AA SequenceMAKPTTLYDKIWNDHLVDEQPDGTCLLYIDRHLVHEVTSPQAFEGLRVAGRKVHAPEKTL AVVDHNVPTTDRSKPNPDPESAEQIAALATNARDFGVQYFNEFDKRQGIVHVIGPEQGFT LPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRAVVDGKLPDGV TAKDIILAIIGEIGTAGGTGYVLEYAGEAIRALTMEGRMTICNMSIEGGARAGLVAPDEK AYEFLKGRPLTPKGANWEQAVRYWDTLRSDDNAHFDHEIRLDAANLPPIVTWGTSPEDVV SVAGFVPDPAKIEDEAKRLSKERALAYMGLNAGTKITDIKLDRVFIGSCTNGRIEDLRAA AKVINGHTVNGHVNAMVVPGSGLVKEQAEAEGLDKIFIKAGFEWREPGCSMCLAMNPDKL APEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWKAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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